Command line ------------------------ The full list of options and what they do can be found on the Command Line Interface (CLI) documentation page: :ref:`Cellpose CLI`. A description of the most important settings can be found on the :ref:`Settings` page. .. _Command line examples: Command Line Usage ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Run ``python -m cellpose`` and specify parameters as below. For instance to run on a folder with images where cytoplasm is green and nucleus is blue and save the output as a png (using default diameter 30): :: python -m cellpose --dir ~/images_cyto/test/ --pretrained_model cyto --chan 2 --chan2 3 --save_png You can specify the diameter for all the images or set to 0 if you want the algorithm to estimate it on an image by image basis. Here is how to run on nuclear data (grayscale) where the diameter is automatically estimated: :: python -m cellpose --dir ~/images_nuclei/test/ --pretrained_model nuclei --diameter 0. --save_png .. warning:: The path given to ``--dir`` must be an absolute path.